Oxford researchers develop 3D printing method that shows promise for repairing brain injuries

This advance marks a significant step towards the fabrication of materials with the full structure and function of natural brain tissues. The work will provide a unique opportunity to explore the workings of the human cortex and, in the long term, it will offer hope to individuals who sustain brain injuries.

Lead author Dr Yongcheng Jin (Department of Chemistry, University of Oxford)

Brain injuries, including those caused by trauma, stroke and surgery for brain tumours, typically result in significant damage to the cerebral cortex (the outer layer of the human brain), leading to difficulties in cognition, movement and communication. For example, each year, around 70 million people globally suffer from traumatic brain injury (TBI), with 5 million of these cases being severe or fatal. Currently, there are no effective treatments for severe brain injuries, leading to serious impacts on quality of life.

Tissue regenerative therapies, especially those in which patients are given implants derived from their own stem cells, could be a promising route to treat brain injuries in the future. Up to now, however, there has been no method to ensure that implanted stem cells mimic the architecture of the brain.

In this new study, the University of Oxford researchers fabricated a two-layered brain tissue by 3D printing human neural stem cells. When implanted into mouse brain slices, the cells showed convincing structural and functional integration with the host tissue.

The cortical structure was made from human induced pluripotent stem cells (hiPSCs), which have the potential to produce the cell types found in most human tissues. A key advantage of using hiPSCs for tissue repair is that they can be easily derived from cells harvested from patients themselves, and therefore would not trigger an immune response.

The hiPSCs were differentiated into neural progenitor cells for two different layers of the cerebral cortex, by using specific combinations of growth factors and chemicals. The cells were then suspended in solution to generate two ‘bioinks’, which were then printed to produce a two-layered structure. In culture, the printed tissues maintained their layered cellular architecture for weeks, as indicated by the expression of layer-specific biomarkers.

Droplets containing human iPSC-derived neural progenitors were 3D-printed to form 2-layer cerebral cortical tissue, which was cultured before implantation into a mouse brain slice. DNPs: deep-layer neural progenitors; UNPs: upper-layer neural progenitors. Image credit: Yongcheng Jin, University of Oxford.

When the printed tissues were implanted into mouse brain slices, they showed strong integration, as demonstrated by the projection of neural processes and the migration of neurons across the implant-host boundary. The implanted cells also showed signalling activity, which correlated with that of the host cells. This indicates that the human and mouse cells were communicating with each other, demonstrating functional as well as structural integration.

3D-printed two-layer cerebral cortical tissue visualised within a mouse brain slice. The implanted neural cells were labelled with fluorescent markers (blue and red in the image). Image credit: Yongcheng Jin, University of Oxford.

The new advance builds on the team’s decade-long track record in inventing and patenting 3D printing technologies for synthetic tissues and cultured cells.

Senior author Dr Linna Zhou (Department of Chemistry, University of Oxford) said: ‘Our droplet printing technique provides a means to engineer living 3D tissues with desired architectures, which brings us closer to the creation of personalized implantation treatments for brain injury.’

Senior author Associate Professor Francis Szele (Department of Physiology, Anatomy and Genetics, University of Oxford) added: ‘The use of living brain slices creates a powerful platform for interrogating the utility of 3D printing in brain repair. It is a natural bridge between studying 3D printed cortical column development in vitro and their integration into brains in animal models of injury.’

Senior author Professor Zoltán Molnár (Department of Physiology, Anatomy and Genetics, University of Oxford) said: ‘Human brain development is a delicate and elaborate process with a complex choreography. It would be naïve to think that we can recreate the entire cellular progression in the laboratory. Nonetheless, our 3D printing project demonstrates substantial progress in controlling the fates and arrangements of human iPSCs to form the basic functional units of the cerebral cortex.'

Senior author Professor Hagan Bayley (Department of Chemistry, University of Oxford) said: ‘This futuristic endeavour could only have been achieved by the highly multidisciplinary interactions encouraged by Oxford's Martin School, involving both Oxford's Department of Chemistry and the Department of Physiology, Anatomy and Genetics.’

Researchers Linna Zhou, Luana Soares, Francis Szele, Zoltán Molnár, and Hagan Bayley are part of the Oxford Martin Programme on 3D Printing for Brain Repair which is working to use 3D printing technologies to generate a low-cost medical technology to address the growing global catastrophe of brain damage through trauma and disease.

The study ‘Integration of 3D-Printed Cerebral Cortical Tissue into an ex vivo Lesioned Brain Slice’ has been published in Nature Communications.